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 BenQ JoyBook Park Ver XXIV, Lookin' Great

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highwind85
post Jun 22 2010, 08:37 AM

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QUOTE(astria @ Jun 22 2010, 08:31 AM)
no laptop to cure ur boredom??? laugh.gif

btw, how come ur iBoiler take so long to come??/
*
Using the 5-year-old iMac in the office...so lyn and facebook is the only hang out place..
don't know..i think it's because the university is buying it...so less priority and more red tape i think...
Office people is working relatively slow...
highwind85
post Jun 22 2010, 09:00 AM

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QUOTE(astria @ Jun 22 2010, 08:50 AM)
haha, maybe...

Australians are not particularly efficient anyway... laugh.gif

hmm.gif, time to clean my laptop... idle at high 40C even at default clcok...
*
Yeah...i have to sent a few emails to push the computing unit person before they ordered it...
Don't feel like writing email to ask when is it coming...
highwind85
post Jun 22 2010, 09:41 AM

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so the white colour squares are the thermal pads?

Added on June 22, 2010, 9:42 ammost probably evo wont be able to sleep tonight..

This post has been edited by highwind85: Jun 22 2010, 09:42 AM
highwind85
post Jun 22 2010, 12:09 PM

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you people on posting spree ar...
@Want, pm me your email...i'll add you later tonight..
highwind85
post Jun 22 2010, 08:03 PM

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me also 64 bit, no problem...
highwind85
post Jun 22 2010, 09:18 PM

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QUOTE(miahahaha @ Jun 22 2010, 08:45 PM)
hmm.gif...no AV installed?? And yet still got this problem?? hmm.gif..

Added on June 22, 2010, 8:54 pmSeems like everyone is getting A42Jv laugh.gif..so much poison, its all whose fault?? laugh.gif
*
A42jv is a good buy ma...some of the missing features are not so much of a deal breaker...14 inch summore...mobility + performance + good price = thumbup.gif
highwind85
post Jun 22 2010, 09:48 PM

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you people ar...spam til so obvious...later mods come and raid baru tau.. sweat.gif
evo definitely wont be able to sleep tonight...how about some world cup? today got 4 matches.. brows.gif
highwind85
post Jun 22 2010, 10:06 PM

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QUOTE(Dackson @ Jun 22 2010, 09:52 PM)
haiz ... wanna reboot and try it out ~
hvnt solve primmer dimmer problem.
It;s possible to use 35% guanidine hydrochloride in PCR prufication kit ? just add it before washing the sample with PE buffer ?? the step is taken from quiaquick kit....
but in the lab there is no guanidine hydrochloride, any alternative chemical compound ?
*
sweat.gif still wanna get rid of the primer dimer...? it doesn't really going to interfere with your results one...
the 3 labs i've been to no people use guanidine hydrochloride...add for what?
How much primer you are using? usually is 1ul of 10uM stock in 50ul/20ul pcr reaction...
highwind85
post Jun 22 2010, 10:15 PM

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QUOTE(Dackson @ Jun 22 2010, 10:12 PM)
using 20 pico molar for 50ul ~
i saw there is list for that chemical compound but can not found the container ~ add to remove primer dimmer in purification. it's under trouble shoot method in quiaquick ~
bcoz need to do cloning ~
*
reduce your primer amount to 10pmol..(Ps: Molar stands for M)
Your primer dimer very bright or what?
highwind85
post Jun 22 2010, 10:21 PM

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France is so dead...France 0-1 SA..

Added on June 22, 2010, 10:25 pmyour PCR is behaving so weird.. sweat.gif
if you just wanna get rid of the primer dimer from the gel photo, just run your gel longer than normal so that the dimers would elute off the gel..they are small in size..could elute much much faster than your products..the dimer is definitely not going to interfere with the next step..usually i'll just ignore it..

Added on June 22, 2010, 10:28 pmFrance red card...

This post has been edited by highwind85: Jun 22 2010, 10:28 PM
highwind85
post Jun 22 2010, 10:46 PM

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QUOTE(Dackson @ Jun 22 2010, 10:30 PM)
not really bcoz primer dimmer size about 100bp so it will shown at the 100bp marker region.
it's weird that after PCR purification, the primer dimmer geting brighter >.<
Supervisor dnt want to see the primer dimmer in the photo/
*
the kit's DNA range is 100bp-10kb...if you primer dimer is about 100bp, the kit might not be able to get rid one...
is it really dimer or a non-specific band of your product?


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highwind85
post Jun 22 2010, 10:58 PM

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QUOTE(evolution120 @ Jun 22 2010, 10:56 PM)
lolx.. btw, which ati modded driver gives the best performance?
*
Just using official drivers... hmm.gif

This post has been edited by highwind85: Jun 22 2010, 10:58 PM
highwind85
post Jun 23 2010, 08:32 AM

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QUOTE(BBXiong @ Jun 23 2010, 08:22 AM)
oh...i finally saw astria on my community map...haha
*
i'll add you later tonight....
@evo,
Feeling restless or not... brows.gif brows.gif
highwind85
post Jun 23 2010, 11:56 AM

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QUOTE(wanttobuylaptop @ Jun 23 2010, 11:47 AM)
iirc, king Aurthur the role playing wargame also force install physx, or is DAO?

my immunet protection factor is 56k...
*
why so high one?
highwind85
post Jun 23 2010, 06:13 PM

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so evo...got reach or not??
highwind85
post Jun 23 2010, 09:06 PM

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4X512mb for 2gb is just waste...if 4X2gb that would be nice...since 4gb is still costing a bomb..
highwind85
post Jun 23 2010, 09:45 PM

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so southeast asians, particularly singaporean, indonesian and malaysian should play JC2....laugh.gif
highwind85
post Jun 23 2010, 10:20 PM

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we are waiting for sxps pictar!!
highwind85
post Jun 24 2010, 12:06 AM

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poor slovenia...thought they would go thru liao...mana tau US scored a last gasp goal..
highwind85
post Jun 24 2010, 05:08 PM

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had an argument with an elite at the other at PhD School...that fella is damn stubborn..
http://forum.lowyat.net/topic/1467634
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