right !!
previously i let it 30min but still like that ><

ok i try it out .. bcoz i just reformat my laptop ~~

><
i format many time ald since i take it out from service center.
I try max OSX once it out from hospital >.<
yesterday i try netbook but some thing to do with GMA make me fail it T.T mac 10.6.3

dling the new version of ccleaner ~~

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Added on June 22, 2010, 8:11 pmi delete it ald ...
wait i do ccleaner 1st ^^

This post has been edited by Dackson: Jun 22 2010, 08:11 PM

here you go ~

the thing keep on not allow me to click on the community ><
now i really felt unsave on it !!

i register ald ..
i can not cancel it at all ><

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Added on June 22, 2010, 8:25 pmi can not click anything on it login acc too ><

This post has been edited by Dackson: Jun 22 2010, 08:25 PM

the problem is once i enter that community it straight pop up that asking me to register, it dnt even allowed me to key in my hotmail or cancel it ... totally freeze at there ><

I'm the person who get this problem ...
that weird.. bcoz i installed it without any AV pre-install ~

right !!
it dnt even shown not responding !!
can not click on anything can not mini size it ==

right ...
now i try to install AV 1st then may give it a last try !! is still like that sorry for it ... i may ban it from my list ^^

huh~
my laptop detected riskware under kaspersky ><

><
wandering why ...
I scared is mine external and pendrive infected by virus bcoz previously helping ppl format (infected with virus one ><)

right

right but only for the community part o~
i can scan, i can click the rest except the community part ><

this also


wandering why ~

This post has been edited by Dackson: Jun 22 2010, 09:40 PM

haiz ... wanna reboot and try it out ~
hvnt solve primmer dimmer problem.
It;s possible to use 35% guanidine hydrochloride in PCR prufication kit ? just add it before washing the sample with PE buffer ?? the step is taken from quiaquick kit....
but in the lab there is no guanidine hydrochloride, any alternative chemical compound ?

it's acer ??

using 20 pico molar for 50ul ~
i saw there is list for that chemical compound but can not found the container ~ add to remove primer dimmer in purification. it's under trouble shoot method in quiaquick ~
bcoz need to do cloning ~

hmm ...
for today 20M it's not very bright !
for me after purification the band look not nice for one of the product. for me it;s look like unspecific binding ><
before i use 5pmolar also can get a bright for one of the product but one pile and 3 no band ><

not really bcoz primer dimmer size about 100bp so it will shown at the 100bp marker region.
it's weird that after PCR purification, the primer dimmer geting brighter >.<
Supervisor dnt want to see the primer dimmer in the photo/

hmmm ...
doc said it's primer dimmer.
bocz previously it's not found in the result dnt know why suddenly appear.
bcoz i reduce the primer concentration end up no primer dimmer lo ~
i found that the PB buffer contain guanidine hydrocloride but the % is unknown. that why need supplement step for it ^^
i think it might be unspecific band or primer dimer for some product. i think 3.0 mM mgcl is quit high !! or may be is my partner pipetting skills problem bcoz she get primer dimer for all the result for today >.<
hope tomorrow still hv DNA sample for me, i wanna try different primer concentration. left 4 samples only. another one i felt i wanna reduce mgcl2 until 2.0 not 3.0 silently ^^

bcoz the primer clump up together make it >50bp lo ~ each primer 20bp only ! non-specific band may bind to the product too together with primer ~

i hvnt rebenchmark it yet ~
why i saw phyX in the driver ?? but i didnt installed any nvidia driver ><

I think no...

Not very sure either come with ATI or window !!
iside hv phyx, nvdia display driver, once deleted dnt know what is the effect after all ><

haiz ...
is my score still half then might be something wrong with the phyX gua ~
i enter save mode delete the immunet but look it it stuck at the cleanup agent there ><
I give it 1 hour run time to clean if not .... T.T

my mini work with the immunet but not mine dv6 ... then something really something inside dv6 kakajiaojiao !!

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Added on June 23, 2010, 1:41 pmhey guy accept me in both email o~
i hv two account, one is netbook and another one is laptop o ~

This post has been edited by Dackson: Jun 23 2010, 01:41 PM