typo.. greece..

haiz ........
dnt know why la ... i try to reinstall the driver when free ... recently not free at all. hving problem with PCR product ><

downloading all x86 and x64 versions of windows 7.. including starter.. lolx

hopefully the hp mini can run win 7 ultimate smoothly..

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Added on June 18, 2010, 2:22 amyes! my dell is at "work in progress" stage! YESSSSSSSS

This post has been edited by evolution120: Jun 18 2010, 02:22 AM

Getting too excited?

some kinda..

late night shock.. off to bed.. nites..

mini can run with win7 ultimate !!
mine install it ^^
eh... just i recommend that dont install the OEM driver just use win7 build in driver for it ! The more driver you install it might eat more ram so it's better to release those ram ! unless you install 2Gb ram ??
that mini wifi weaker than bigger laptop o ~ it detect 1 level but mine dv detect as 3 level ><
all mine accidentally deleted !!

So, evo laptop already have progress

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Added on June 18, 2010, 7:34 amJust visited Broadband User 2 User sub-section, bro Sordanny got one post there currently ...

This post has been edited by miahahaha: Jun 18 2010, 07:34 AM

morning ~

i thought u guys know my netbook is running on Win7 Home Premium x64???

N450 + 1GB... does some decent folding too...

what's wrong with your PCR product?

I know ...
T2400 can fold why not atom ??
why dnt use win7 ultimate x64 ??
actually since the ram max until 2gb it's a waste to use x64 bcoz x86 ald maximize it ald lo ~

with double band, some time the band is pile in color, or primer dimer, smear too ><
sample using 1.5mM MgCl, 2.0 MgCl, and 3.0 MgCl std formula ~.~ weird i get bright band in optimization but i get pile band in normal one using same formula same temperature >.<
after change the buffer and MgCl, the same thing happen again @.@
You know how to use nanophotometer to determine DNA concentration manually ? the machine hv something wrong ><

This post has been edited by Dackson: Jun 18 2010, 08:41 AM

wasteful la... netbook use Ultimate...

use x64 mainly all the computers that i ve are all running on x64.. easier to maintain...

What type of DNA sample are you trying to PCR? Genomic DNA? Is the temperature for the primers optimized? Don't worry about primer dimers...usually is caused by high concentration of primers used..
Manually? Get the A260 reading and convert it to concentration..A260 of 1.0 = 50µg/ml pure DNA. try it on a different spectrophotometer if you doubt the machine..

for me it's the same, i install win7 pro version too ...
luckily mine hv x86 and x64 for software ^^
just looking for linux one ^^ linux save me twice ald ...

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Added on June 18, 2010, 9:02 amit's double stranded DNA.
bcoz supervisor dnt like to see dimer and pile band T.T
right the primer Ta is optimize ald !! today will run purification for it, but i think double band still exist. If not mistaken it's loading dye problem !
If not really no idea, if wanna change all again ... ><
A260/A280 for DNA and A260/A230 for RNA but need both for more accurate ! bcoz still hv lid factor, length of optical path that all, all those thing influence the concentration >< nanophotometer is different from spectrophotometer ><
i get 0.090 ug/ml for pure DNA but A260/A280give 2.0 which is the best result, A260/A230 is 6.0 which shown there is very little amount of RNA containminated.
smear can be result by incomplete DNA product, RNA and dimer or even MgCl : DNTP ratio, thinking to add some KCl, DmSo but i dnt think they allow me to do that due to I'm still undergraduate >.<
bcoz i get nice band for optimization, but can not get back the nice band back weird ... without purfication the band ald bright and no smear ....
May be need to change taq ?? PFU band is ok but still hv some band not bright enough that mean their optimum mixture is not really optimum ??
if still like that may be need to extract another DNA sample again ??

This post has been edited by Dackson: Jun 18 2010, 09:05 AM

What is your double stranded DNA? Genomic or plasmid DNA? Dont like dimer then decrease the primer concentration, or run your gel longer so that the dimers would be eluted..
0.090ug/ml is god damn little dude...0.090 ng/ul is almost equal to nothing..i would not take anything less than 10ng/ul..
Normal Taq should do the job...Pfu is proof-reading taq..How long is your expected insert? and what do you plan to do with the amplified DNA later? if your expected insert is more than 1.5kb and going to use it for protein expression, then might be a good idea to use Pfu...if not just waste money ny..

lol apa ini?

that's a lot of stars...

Biology class

Previously got programming class also...

@Dackson
If you still wanna convert the DNA concentration yourself, this is the equation:

c = (A * e)/b
Where c is the nucleic acid concentration in ng/microliter, A is the absorbance in AU, e is the wavelength-dependent extinction coefficient in ng-cm/microliter and b is the path length in cm. The generally accepted extinction coefficients for nucleic acids are:
• Double-stranded DNA: 50
• Single-stranded DNA: 33
• RNA: 40
For the NanoDrop® ND-1000 Spectrophotometer,paths of 1.0 mm and 0.2 mm are used compared to a standard spectrophotometer using a 10.0 mm path..

but i personally don't think it will make a difference..using other spectro or gel quantification would be a better option..

This post has been edited by highwind85: Jun 18 2010, 09:49 AM

sure is multiple thread xD~
sometime hv networking class too ^^